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Bridge PTS

Home
Our Company
About
Why Bridge PTS?
Getting Started
Collaborators
Licenses
BRIDGE PTS Cited Publications
Services
Wound Healing
Infection Control & Biofilms
Customized Surgical Work
Our Facility
Posters
Events
Blog
Contact Us
Search
  • Biofilm & Microbiology Testing
  • Rodent Lung Infection Animal Model
  • Rodent Implantable Biofilm Coated Devices
  • Rabbit Foley Catheter/Urinary Infection
  • Minimum Inhibitory Concentration (MIC) / Minimum Bactericidal Concentration (MBC)
  • Time Kill (TK)
  • Zone of Clearance/Inhibition (ZOC/ZOI)
  • Simple (Single Species) Biofilms
  • Complex (Multi-Species) Biofilms
  • Live-Dead Staining
  • Electron Microscopy
  • Tensile Testing (BF)
  • Rabbit Peritoneal Implant Model
  • Rabbit Vaginal Mucosal Irritation Model

Rodent Implantable Biofilm Coated Devices

  • Duration of study – Up to 6 months

  • Lead time – 30 days

  • Standard results – Microbiology, Histology


Model utility:

The rat subcutaneous implant model is our most frequently requested (and most frequently recommended) animal model for screening antimicrobial and anti-biofilm actives and coatings. The model is fast, relatively inexpensive and, in our experience, 75-80% predictive of more complicated animal models. Using this model, it is possible to screen a wide array of microbial species with high reproducibility. Furthermore, as with all infection models, results are fast and unambiguous – actives that work generate significant log reductions compared to controls.

How the Model Works:

  • Device is implanted subcutaneously (typically a polypropylene hernia mesh)

  • Infection is added through precultured of the biofilm onto the material prior to implantation

  • Commonly requested bacteria are: MRSA, S. epidermidis, E. coli, and Pseudomonas

  • Treatment can begin any time before or after implantation

  • Implanted device and surrounding tissue are collected for microbial counts on both selective and non-selective media.

  • The surrounding tissue can also be evaluated histologically

  • Study duration can be hours up to 6 months

Example Data:

This data was generated from a model development study that assessed bioburden after inoculation of the test implants with various concentrations of Staphylococcus aureus and incubation for 30 minutes. 

Figure 1.  A comparison of total bioburden present on a silicone mesh inoculated with varying concentrations of S. aureus.  Meshes were sampled at Day 7 and compared to control meshes that were not implanted and were sampled on Day 0.

Figure 1.  A comparison of total bioburden present on a silicone mesh inoculated with varying concentrations of S. aureus.  Meshes were sampled at Day 7 and compared to control meshes that were not implanted and were sampled on Day 0.

Figure 2. A comparison of total Staphylococcus colony forming units (CFUs) present on a silicone mesh inoculated with varying concentrations of S. aureus. Meshes were sampled at Day 7 and compared to control meshes that were not implanted and were s…

Figure 2. A comparison of total Staphylococcus colony forming units (CFUs) present on a silicone mesh inoculated with varying concentrations of S. aureus. Meshes were sampled at Day 7 and compared to control meshes that were not implanted and were sampled on Day 0.

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BRIDGE PTS, Inc., Brooks City-Base, San Antonio, TX 78235, P: 210-532-7344